|
KMID : 0043320140370060803
|
|
Archives of Pharmacal Research
2014 Volume.37 No. 6 p.803 ~ p.812
|
|
|
Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells
|
|
Shin Dong-Mi
Choi Kyeong-Mi
Lee Youn-Sun
Kim Won-Kyun
Shin Kyong-Oh
Oh Sei-Kwan
Jung Jae-Chul
Lee Mi-Kyeong
Lee Yong-Moon
Hong Jin-Tae
Yun Yeo-Pyo
Yoo Hwan-Soo
|
|
|
Abstract
|
|
|
|
Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1-methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.
|
|
|
KEYWORD
|
|
|
Echinacea purpurea, 3T3-L1 cells, Adipocyte differentiation, Insulin, Dodeca-2(E), 4(E)-dienoic acid isobutylamide
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|